Skip Navigation


International Immunology Advance Access originally published online on April 7, 2008
International Immunology 2008 20(5):709-718; doi:10.1093/intimm/dxn027
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
20/5/709    most recent
dxn027v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Palazzo, M.
Right arrow Articles by Rumio, C.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Palazzo, M.
Right arrow Articles by Rumio, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?


© The Japanese Society for Immunology. 2008. All rights reserved. For permissions, please e-mail: journals.permissions@oxfordjournals.org

Cross-talk among Toll-like receptors and their ligands

Marco Palazzo1, Silvia Gariboldi1, Laura Zanobbio2, Giuseppina F. Dusio1, Silvia Selleri1, Marzia Bedoni1, Andrea Balsari2,3 and Cristiano Rumio1

1 Department of Human Morphology
2 Institute of Pathology, Università degli Studi di Milano, via Mangiagalli 31, 20133 Milan, Italy
3 Department of Experimental Oncology Fondazione IRCCS, Istituto Nazionale Dei Tumori, via Venezian 1, 20133 Milan, Italy

Correspondence to: C. Rumio; E-mail: cristiano.rumio{at}unimi.it

Toll-like receptors (TLRs) 4, 5, 7 and 9 belong to a family of proteins that recognize mainly conserved microbial motifs. Though each TLR has a highly specific ability to recognize a particular microbial pattern, recent papers suggest that some ligands are able to affect the expression of different TLRs. In this paper, we have investigated TLR4, 5, 7 and 9 expression, both at mRNA and protein level, following treatment of different intestinal epithelial cell lines with LPS, flagellin, loxiribine, CpG-oligodeoxynucleotide and peptidoglycan, to assess if the different TLR ligands may modulate the expression of the respective TLR and of the unrelated ones. Our results show that a cross-talk exists between TLRs and various ligands, indicating a cross-regulation among these pattern recognition receptors. In particular, TLR4 was generally down-regulated by treatment with ligands other than LPS, while flagellin and unrelated microbial-associated molecular patterns exerted a general stimulatory activity as regards TLR5 expression. Concerning TLR7 and 9, we have observed a more variable behaviour of the various cell lines with the different ligands. Together, our results demonstrate that the expression of TLRs in intestinal cells is highly dynamic and tightly regulated in response to encountered microbial stimuli.

Keywords: CpG-ODN, flagellin, loxiribine, LPS, peptidoglycan

Transmitting editor: G. Trinchieri

Received 27 November 2007, accepted 19 February 2008.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.